About

Translation of Tissue Engineering Technologies: Tissue engineering is rapidly growing as an alternative for the repair and regeneration of lost or damaged organs and tissue. As further advances are made, academic and industrial researchers are beginning the shift towards translating and commercializing these exciting discoveries and technologies. Some of these products are basic building blocks, the tools and materials used by engineers, while others are combinations of technologies that make the make the products that will make it to the bedside. This symposium will provide an opportunity for tissue engineers to provide valuable input for translating unique technologies from the bench to the bedside. Topics could include, but are not limited to, developing technologies/products to be used by engineers, designing in vitro and in vivo studies aimed at translating technologies, advanced preclinical and clinical models, approaching the FDA, and regulatory strategy as it impacts research and business efforts.

Abstracts

1:00 p.m. 1. Development and application of electrospun supramolecular absorbable medical devices in preclinical to clinical trials, M. Cox*(1), M. Brugmans(1), F. Zomer Volpato(1), O. Svanidze(1), F. Schoen(2), (1)Xeltis, Eindhoven, Netherlands, (2)Brigham and Women's Hospital, Boston, MA
1:15 p.m. 2. Brain hydrogel delivery of clustered Vascular Endothelial Growth Factor promotes behavioral recovery after stroke, L. R. Nih*, T. segura, ucla, los angeles, CA
1:30 p.m. 3. In Situ Fibrillizing Collagen Solutions for Soft Tissue Augmentation, D. DeVore*(1), M. Collins(1), D. Grant(2), S. A. Grant(2), (1)EternoGen, LLC, Columbia, MO, (2)University of Missouri, Columbia, MO
1:45 p.m. 4. Effect of hormone levels, gender, body mass index, age and life-style parameters on human craniofacial bone regeneration with tricalcium phosphate grafts, C. Knabe*(1), A. Mele(1), P. H. Kann(1), B. Peleska(1), D. Adel-Khattab(2), H. Renz(1), A. Reuss(1), M. Bohner(2), M. Stiller(1), (1)Philipps University Marburg, Marburg, Germany, (2)Ais Shams University, Cairo, Egypt
2:00 p.m. 5. Cell-Seeded Synthetic Scaffold for Esophageal Regeneration, S. Soliman*, M. Marsh, J. Laurent, S. La Francesca, Biostage, Holliston, MA
2:15 p.m. 6. Electrical application on titanium implants stimulating bone growth, L. M. Bins-Ely(1), C. A. M. Benfatti(1), B. Henriques(1), M. T. Mathew(2), R. S. Magini(1), J. C. M. Souza*(1), (1)Federal University of Santa Catarina (UFSC), Florianópolis, Brazil, (2)University of Illinois, Rockford, IL
2:30 p.m. 7. A New Treatment Option for Fetal Aqueductal Stenosis: Ventriculo-Amniotic Shunt, P. Shridhar*, Y. Chen, S. Greene, S. Emery, Y. Chun, University of Pittsburgh, Pittsburgh, PA
2:45 p.m. 8. Woven Collagen Biotextiles for Rotator Cuff Tendon Repair - An In Vivo Pilot Investigation, G. D. Learn*(1), P. E. McClellan(1), D. M. Knapik(2), J. L. Cumsky(1), R. J. Gillespie(2), O. Akkus(1), (1)Case Western Reserve University, Cleveland, OH, (2)University Hospitals Case Medical Center, Cleveland, OH

Acellular Biomaterials for Myocardial Repair 1

About

Acellular biomaterials for myocardial repair: Using biomaterials without exogenous cells for myocardial repair has attracted great attention in the past several years largely due to their high translational potential. These biomaterials are designed to possess one or more of the following functions: provide mechanical support to the damaged hearts, prevent cardiac extracellular matrix from degradation by upregulated MMPs, recruit endogenous cells for regeneration, stimulate vascularization in the infarcted area, control inflammation to promote survival of cardiac cells, and decrease cardiac fibrosis. These biomaterials include injectable hydrogels, microspheres, nanoparticles, and 3D scaffolds. The injectable hydrogels, microspheres, and nanoparticles can be delivered to the infarcted hearts by localized injection after open chest surgery, catheter-based injection, or intravenous injection. Acellular 3D scaffolds can be patched on the infarcted heart surface. The biomaterials alone or in combination with drugs, genes, peptides, and growth factors have been used to promote cardiac repair. This symposium will allow presenters to share their current advances in using acellular biomaterials for cardiac repair.

Abstracts

1:00 p.m. 9. Invited Speaker: William Wagner, PhD - Design of Thermoresponsive Hydrogels and Biodegradable, Thermoplastic Elastomers for Interventions in Cardiac Wall Remodeling Following Myocardial Infarction, W. R. Wagner*, Y. Zhu, X. Gu, A. D'Amore, Y. Matsumura, McGowan Institute for Regenerative Medicine, Pittsburgh, PA
1:30 p.m. 11. Therapeutic Microparticles Functionalized with Biomimetic Cardiac Stem Cell Membranes and Secretome, J. Tang(1), A. Vandergriff(1), T. Allen(1), M. Hensley(1), J. Cores(1), P.-U. Dinh(1), J. Zhang(2), K. Cheng*(1), (1)North Carolina State University, Raleigh, NC, (2)zhengzhou university, zhengzhou, China
1:45 p.m. 12. Transcatheter Tissue-Engineered Vein Valve, Z. Syedain*, C. Feagler, T. Le, R. Tranquillo, University of Minnesota, Minneapolis, MN
2:00 p.m. 13. Dual Delivery of TGF? Receptor 2 Binding Peptide and Oxygen to Control Cardiac Fibrosis, Z. Fan, M. Fu, J. Guan*, The Ohio State University, Columbus, OH
2:15 p.m. 14. Developing dual-delivery nanogels for treatment of myocardial infarction, E. P. Mihalko*(1), E. P. Sproul(1), K. Cheng(1,2), A. C. Brown(1), (1)North Carolina State University and University of North Carolina at Chapel Hill, Raleigh, NC, (2)North Carolina State University College of Veterinary Medicine, Raleigh, NC
2:30 p.m. 15. Injectable acellular guest-host hydrogel for miR-302 delivery promotes cardiomyocyte proliferation, L. L. Wang*(1), Y. Liu(2), A. C. Gaffey(3), J. J. Chung(3), P. Atluri(3), E. E. Morrisey(1), J. A. Burdick(1), (1)University of Pennsylvania, Philadelphia, PA, (2)Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, (3)Hospital of th
2:45 p.m. 16. Invited Speaker: Karen Christman, PhD

Invited Speaker(s)

William Wagner, PhD
Karen Christman, PhD

Biomaterials for Regenerative Engineering 1

About

Biomaterials for Regenerative Engineering: Regenerative engineering aims to develop functional, bioactive, and instructive biomaterials for regeneration of damaged or injured tissues. Bioactivity can be engineered into biomaterials by functionalization with proteins, peptides, small molecules as well as by biophysical cues such as surface topography or alignment. This symposium will highlight recent trends in development of bioactive biomaterials that play active role in controlling cellular behaviors such as growth, alignment and differentiation. We will include different classes of biomaterials such as proteins, polysaccharides, synthetic polymers, fibers, metals, ceramics, and hydrogels for applications in regenerative tissue engineering. Translational strategies for taking these biomaterials from ‘Bench to Bedside’ will also be discussed during the symposium.

Abstracts

1:00 p.m. 17. Invited Speaker: Cato Laurencin, MD, PhD ,
1:30 p.m. 19. Porous Hydrogel Patch Seeded with Stem Cells for Treating Alveolar Air Leaks, G. Vunjak-Novakovic*, B. Guenthart, J. O'Neill, J. Kim, M. Bacchetta, Columbia University, New York, NY
1:45 p.m. 20. Immune Modulatory Biomaterials For Cell Based Therapies, O. Veiseh*, Sigilon, Cambridge, MA
2:00 p.m. 21. Injectable and Resorbable Nanocrystalline-poly(ester-urethane) Bone Cements Exhibit Bone-like Strength and Enhance Cellular Activities, S. Lu*(1), M. A. P. McEnery(1), B. R. Rogers(1), D. Shimko(2), J. C. Wenke(3), S. A. Guelcher(1), (1)Vanderbilt University, Nashville, TN, (2)Medtronic Spine and Biologics, Memphis, TN, (3)Orthopaedic Task Area, U.S. Army Institute of Surgical Research, S
2:15 p.m. 22. Tuning Heterogeneities into Photoclickable Synthetic Hydrogels for Improved Cartilage Tissue Engineering, M. Schneider*, S. Chu, S. Lalitha Sridhar, F. Vernerey, S. Bryant, University of Colorado, Boulder, Boulder, CO
2:30 p.m. 23. Engineering Pre-Vascularized Skeletal Muscle with Physiologically-Relevant Cellular Organization for Treatment of Volumetric Muscle Loss, N. F. Huang*(1), K. H. Nakayama(1), M. Quarta(2), V. Garcia(2), Z. Strassberg(2), I. Akimenko(3), O. Abilez(1), T. A. Rando(2), (1)Stanford University, Stanford, CA, (2)Stanford University, Palo Alto, CA, (3)Veterans Affairs Palo Alto Health Care System,
2:45 p.m. 24. Harnessing sphingosine-1-phosphate signaling and nanotopographical cues to regulate skeletal muscle maturation and vascularization, J. H. Tsui(1), K. Janebodin(1), N. Ieronimakis(1), D. M. P. Yama(1), H. S. Yang(2), R. Chavanachat(1), A. L. Hays(1), M. Reyes(3), D.-H. Kim*(1), (1)University of Washington, Seattle, WA, (2)Dankook University, Cheonan, Republic of Korea, (3)Albert Einstein College of Medicine, Bronx, NY

Invited Speaker(s)

Cato T. Laurencin, MD, PhD

Biomaterials with Dynamic Properties

About

Biomaterials with Dynamic Properties: Hydrogels with dynamic biophysical and biochemical properties are increasingly used to recapitulate changes seen in the native extracellular matrix or to facilitate cellular remodeling of the matrix. This session will focus on the design principles and characterization of biomaterials with time-dependent properties (e.g., mechanics) imparted by dynamic covalent, physical, or stimuli-responsive interactions. Applications discussed will include, but are not limited to, cellular mechanosensing, differentiation, and dynamic ligand presentation. The use of these platforms for controlled biomolecule delivery will also be included.

Abstracts

1:00 p.m. 25. Engineering Dynamic Reversible PEG Hydrogels using Light-sensing Proteins, J. A. Hammer*, R. M. Schweller, J. L. West, Duke University, Durham, NC
1:15 p.m. 26. Dynamic and Reversible Stiffness Modulation in Photoresponsive Protein-Polymer Hydrogels, L. Liu, J. A. Shadish, C. A. DeForest*, University of Washington, Seattle, WA
1:30 p.m. 27. Withdrawn
1:45 p.m. 28. Enzyme-Mediated Stiffening Hydrogels for Studying Pancreatic Cell Malignancy, H.-Y. Liu*(1), C.-C. Lin(1,2), (1)Purdue University, Indianapolis, IN, (2)Indiana University, Indianapolis, IN
2:00 p.m. 29. Modulation of Cellular Response Using Mechanically Dynamic PDMS Substrates, Y.-C. Yeh*(1), S. Caliari(1), S. Vega(1), L. Ouyang(2), L. Han(2), J. Burdick(1), (1)University of Pennsylvania, Philadelphia, PA, (2)Drexel University, Philadelphia, PA
2:15 p.m. 30. Amplified Photodegradation of Cell-laden Hydrogels Through an Addition-Fragmentation Chain Transfer Reaction, T. E. Brown*, I. Marozas, K. S. Anseth, University of Colorado - Boulder, Boulder, CO
2:30 p.m. 31. Shape Changing Photodegradable Hydrogels for 2D to 3D Cell Culture, S. M. Delgado*, A. M. Kasko, UCLA, Los Angeles, CA
2:45 p.m. 32. Temperature-Controlled Capture and Release of Heparin-Binding Proteins and Cells on Heparin-Immobilized Thermoresponsive Cell Culture Substrates, J. Kobayashi*, Y. Akiyama, M. Yamato, T. Okano, Tokyo Women's Medical University, Tokyo, Japan

Cell Migration and Biomaterials

About

Cell Migration and Biomaterials: Cell migration is a crucial component in the development, remodeling, and repair of healthy tissue. As engineers and biologists develop a better understanding of how the local microenvironment influences cell migration, we can design materials that better control cell migration for a wide range of applications, including tissue engineering, cancer, and cell delivery. With this in mind, this session focuses on the influence of biomaterials on cell migration. Appropriate papers include exploring the experimental and/or theoretical aspects of cell migration in two or three dimensional materials, as well as the fundamental mechanisms of cell-matrix/surface interactions. Papers may also include studies on the role of chemokine expression and/or delivery in cell migration, the determination of critical biomaterial design parameters for controlled cell migration, and the role of physical and chemical biomaterial properties on cell migration. Applications may vary from controlling cancer cell invasion to directed cell migration for tissue engineering, among others.

Abstracts

1:00 p.m. 33. Invited Speaker: Cell Migration in Confined Microenvironments: Stiffness Matters, Konstantinos Konstantopoulos, PhD
1:30 p.m. 35. Effect of Nuclear Softening and Fiber Stiffness on Cell Migration into Dense Fibrous Networks, K. Song*, S.-J. Heo, M.-H. Lee, R. L. Mauck, J. A. Burdick, University of Pennsylvania, Philadelphia, PA
1:45 p.m. 36. Non-swelling microfluidic hydrogels reveal that matrix degradability controls collectivity of angiogenic invasion, B. M. Baker*(1), B. Trappmann(2), J. A. Burdick(3), C. S. Chen(4), (1)University of Michigan, Ann Arbor, MI, (2)Max Planck Institute, Munster, Germany, (3)University of Pennsylvania, Philadelphia, PA, (4)Boston University, Boston, MA
2:00 p.m. 37. 3D Tumor Model to Investigate Natural Killer Cell-Cancer Cell Interactions, I. Adjei*(1), G. Plumton(1), J. Djeu(2), B. Sharma(1), (1)University of Florida, Gainesville, FL, (2)Moffitt Cancer Center, Tampa, FL
2:15 p.m. 38. Single Cell Migration Speed on Nanofibers is Rho/ROCK and Focal Adhesion Structure Dependent, D. T. Bowers*, J. L. Brown, The Pennsylvania State University, University Park, PA
2:30 p.m. 39. Micropatterns Enhance Endothelial Cell Migration Under Flow Conditions, L. A. Blume(1), J. R. Strom(1), R. B. Dodson(2), A. B. Brennan(1,3), C. M. Magin*(1,4), (1)Sharklet Technologies, Inc., Aurora, CO, (2)University of Colorado, Anschutz Medical Campus, Aurora, CO, (3)University of Florida, Aurora, CO, (4)University of Colo
2:45 p.m. 40. Microgel Thin Films Direct Cell Spreading and Migration Responses, D. Chester*, J. Nortey, R. Kathard, A. C. Brown, North Carolina State University, Raleigh, NC

Invited Speaker(s)

Konstantinos Konstantopoulos, PhD

Emerging Applications in Engineering Cells and Their Microenvironments

About

Emerging Applications in Engineering Cells and Their Microenvironments: There has been an emergence of multiple techniques to engineer cells and their microenvironments in order to control a wide range of cell behaviors. This session will focus on translation of these techniques towards specific applications ranging from organ-on-chip and diagnostic assays to tissue engineering for disease models and regenerative medicine.

Abstracts

1:00 p.m. 41. Mineralized Collagen Matrices Increase Breast Cancer Bone Metastatic Potential, S. Choi*(1), J. Friedrichs(2), C. Werner(2), L. Estroff(1), C. Fischbach(1), (1)Cornell University, Ithaca, NY, (2)Leibniz Institute of Polymer Research, Dresden, Germany
1:15 p.m. 42. Contractile abnormalities induced by biomechanical mismatch in human iPS-based cardiac microtissues, Z. Ma*(1), S. Koo(2), P. Hoang(1), N. Huebsch(2), B. Conklin(3), C. Grigoropoulos(2), K. Healy(2), (1)Syracuse University, Syracuse, NY, (2)University of California, Berkeley, Berkeley, CA, (3)Gladstone Institute of Cardiovascular Disease, San Francisco,
1:30 p.m. 43. Serum from Transcatheter Aortic Valve Replacement Patients Reveals Links to Valvular Interstitial Cell Activation, B. A. Aguado*(1), K. B. Schuetze(2), J. C. Grim(1), T. A. McKinsey(2), K. S. Anseth(1,3), (1)Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO, (2)University of Colorado - Anschutz Medical Campus, Aurora, CO, (3)BioFrontiers Ins
1:45 p.m. 44. Design of multidimensional culture systems for probing the role of integrin binding in fibroblast activation, M. E. Smithmyer*, A. M. Kloxin, University of Delaware, Newark, DE
2:00 p.m. 45. Integrin Ligand-specific, Lymphoid Niches to Study Lymphoma Tumors, S. B. Shah*(1), Y. F. Tian(1), L. Cerchietti(2), E. Cesarman(2), A. Singh(1), (1)Cornell University, Ithaca, NY, (2)Weill Cornell Medical College, New York, NY
2:15 p.m. 46. Enhanced Vascularization of the Subcutaneous Space using a Vascular Regenerative Biomaterial, V. F. Coindre*, M. V. Sefton, University of Toronto, Toronto, ON
2:30 p.m. 47. Vasculogenic Degradable Hydrogel to Enhance Islet Engraftment, Function, and Survival within Extrahepatic Transplant Sites, J. D. Weaver*(1), D. M. Headen(1), J. Aquart(1), C. T. Johnson(2), A. J. Garcia(1), (1)Georgia Institute of Technology, Atlanta, GA, (2)Emory-Georgia Institute of Technology, Atlanta, GA
2:45 p.m. 48. Non-genetic, Transient Engineering of Mesenchymal Stem Cell Secretome via Intracellular Controlled Drug Delivery, S. H. Ranganath*(1), Z. Tong(2), O. Levy(2), K. Martyn(2), J. M. Karp(2), M. S. Inamdar(3), (1)Siddaganga Institute of Technology, Tumkur, India, (2)Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, (3)Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India

Nucleic Acid Delivery

About

Nucleic Acid Delivery: While the majority of sequences in the genome and RNAs in the transcriptome have the potential to be modulated for therapeutically using exogenous nucleic acids, effective and safe delivery remains a technical barrier to clinical translation. Thus, the design of vehicles to deliver nucleic acids to target cells in a safe and effective manner is a rapidly evolving area within the biomaterials field. In addition to continued advances in more established areas such as nonviral gene therapy (i.e.,delivery of plasmid DNA), new approaches are also emerging for delivery of siRNA, mRNA, microRNA, and CRISPR-Cas9. This symposium will highlight recent progress on cutting edge biomaterials systems including polymers, lipids, conjugates, and multicomponent nanoparticle platforms for design and delivery of siRNA, antisense, micro-RNA, micro-RNA inhibitors (i.e., PNA, LNA, etc.), and plasmid DNA. Abstracts on research related to epigenetics, micro-RNA regulation, mRNA-based immunotherapies, and delivery of RNA-guided genome editing technologies are especially sought.

Abstracts

1:00 p.m. 49. Invited Speaker: Nucleic acid delivery systems for RNA therapy and gene editing, Dan Anderson, PhD
1:30 p.m. 51. Functional polyesters enable selective small RNA delivery to orthotopic lung tumor cells over normal cells, D. Siegwart*, University of Texas Southwestern Medical Center, Dallas, TX
1:45 p.m. 52. In Vivo Nanoparticle-Mediated RNAi in Bone Marrow Enhances Hematopoietic Stem Cell Mobilization and Harvesting, M. J. Mitchell*, R. Langer, MIT, Cambridge, MA
2:00 p.m. 53. Delivery of MicroRNA Using L-tyrosine Polyurethane Nanoparticles as a Therapy for Liver Fibrosis, J. Hyun(1), S. Wang(1), J. Kim(1), K. M. Rao(1), S. Y. Park(1), I. Chung(1), C.-S. Ha(1), S.-W. Kim(1), Y. Jung(1), Y. H. Yun*(2), (1)Pusan National University, Busan, Republic of Korea, (2)University of Akron, Akron, OH
2:15 p.m. 54. Utilizing Injectable Decellularized Extracellular Matrix Hydrogels for the Slow Release of MicroRNAs, M. J. Hernandez*(1), R. Gaetani(1), J. L. Ungerleider(1), J. P. G. Sluijter(2), K. L. Christman(1), (1)University of California, San Diego, La Jolla, CA, (2)University Medical Center, Utrecht, Netherlands
2:30 p.m. 55. Polyethylenimine-Coated Spherical Nucleic Acid Nanoparticles as Efficient, Low Dose Gene Regulatory Agents, J. R. Melamed*, N. L. Kreuzberger, E. S. Day, University of Delaware, Newark, DE
2:45 p.m. 56. Nanotherapeutics for Combination Drug and Gene Therapy in Treating Glioblastoma Multiforme, A. A. Alexander-Bryant*(1), B. Hourigan(1), M. Lynn(2), J. S. Lee(1), (1)Clemson University, Clemson, SC, (2)Greenville Health System, Greenville, SC

Invited Speaker(s)

Dan Anderson, PhD

Orthopedic Biomaterials 1

Abstracts

1:00 p.m. 57. Uniaxial BCP/Collagen Bi-layered Scaffold for Osteochondral Regeneration by Stem Cells Migration, S.-H. Jeong, H.-E. Kim, Seoul national university, Seoul, Republic of Korea
1:15 p.m. 58. Electrochemical induced cell death of monocyte inflammatory cells on CoCrMo alloy in vitro, M. J. Wiegand*, G. W. Kubacki, J. L. Gilbert, Syracuse University, Syracuse, NY
1:30 p.m. 59. Molecular Engineering of Arthritic Human Cartilage Ex Vivo Using Biomimetic Proteoglycans, E. Phillips*, N. Bertha, B. Shallop, K. Prudnikova, M. Mulcahey, M. Marcolongo, Drexel University, Philadelphia, PA
1:45 p.m. 60. The Prevalence of a Column-Like Damage Pattern within CoCrMo Femoral Head Tapers, D. J. Hall*, R. M. Urban, S. M. McCarthy, J. L. Wright, J. J. Jacobs, R. Pourzal, Rush University Medical Center, Chicago, IL
2:00 p.m. 61. Withdrawn
2:15 p.m. 62. Novel Antibacterial Coating on Orthopedic Implants, D. Gil*, A. Frank-Kamenetskii(1), V. Reukov(1), C. Gross(2), A. Vertegel(1), (1)Clemson University, Clemson, SC, (2)Medical University of South Carolina, Charleston, SC
2:30 p.m. 63. Final results of a clinically relevant bone regeneration study in the goat calvaria model, S. Chen, O. Ortiz*, J. Kohn, Rutgers University, Piscataway, NJ
2:45 p.m. 64. Microarchitecture Parameters Influencing Bone Mineralization In An In Vitro Study, JA. Pura*(1), M. Pellicelli(2), R. St. Arnaud(2), D. Pasini(1), L. Haglund(1), M. Tanzer(1), (1)McGill University, Montreal, QC, (2)Shriners Hospitals for Children-Canada, Montreal, QC

*BTI* Translation of Tissue Engineering Technologies

About

Abstracts

Acellular Biomaterials for Myocardial Repair 1

About

Abstracts

Invited Speaker(s)

Biomaterials for Regenerative Engineering 1

About

Abstracts

Invited Speaker(s)

Biomaterials with Dynamic Properties

About

Abstracts

Cell Migration and Biomaterials

About

Abstracts

Invited Speaker(s)

Emerging Applications in Engineering Cells and Their Microenvironments

About

Abstracts

Nucleic Acid Delivery

About

Abstracts

Invited Speaker(s)

Orthopedic Biomaterials 1

Abstracts

BTI Translation of Tissue Engineering Technologies